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ATCC rat embryonic heart derived h9c2 cells
Isoproterenol depletes intracellular iron in <t>H9c2</t> cells. Analysis of intracellular iron levels from the cells challenged with isoproterenol and/or deferoxamine. Data are expressed as mean ± SEM; (*** p < 0.001 vs. control).

Isoproterenol depletes intracellular iron in <t>H9c2</t> cells. Analysis of intracellular iron levels from the cells challenged with isoproterenol and/or deferoxamine. Data are expressed as mean ± SEM; (*** p < 0.001 vs. control).

Rat Embryonic Heart Derived H9c2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3769 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat embryonic heart derived h9c2 cells - by Bioz Stars, 2026-07

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1) Product Images from "Iron Matters: Comparative Impact of Beta-Adrenergic Stimulation and Iron Chelation on Cardiac Iron Metabolism and Mitochondrial Function"

Article Title: Iron Matters: Comparative Impact of Beta-Adrenergic Stimulation and Iron Chelation on Cardiac Iron Metabolism and Mitochondrial Function

Journal: Biomolecules

doi: 10.3390/biom16040582

Isoproterenol depletes intracellular iron in H9c2 cells. Analysis of intracellular iron levels from the cells challenged with isoproterenol and/or deferoxamine. Data are expressed as mean ± SEM; (*** p < 0.001 vs. control).

Figure Legend Snippet: Isoproterenol depletes intracellular iron in H9c2 cells. Analysis of intracellular iron levels from the cells challenged with isoproterenol and/or deferoxamine. Data are expressed as mean ± SEM; (*** p < 0.001 vs. control).

Techniques Used: Control

Isoproterenol modifies iron metabolism-related molecules in H9c2 cells. Analysis of mRNA ( a , c , e , f ) and protein levels ( b , d , g , h ) of IRP1 ( Aco1 ) ( a , b ), IRP2 ( Ireb2 ) ( c , d ), Fth1 ( e ), Ftl1 ( f ) and FERRITIN ( g ). Representative Western blot image of all the analyzed proteins ( h ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or by ACTIN protein levels and expressed as mean ± SEM; (* p < 0.05, ** p < 0.01 and *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. ISO; && p < 0.01 vs. DEF).

Figure Legend Snippet: Isoproterenol modifies iron metabolism-related molecules in H9c2 cells. Analysis of mRNA ( a , c , e , f ) and protein levels ( b , d , g , h ) of IRP1 ( Aco1 ) ( a , b ), IRP2 ( Ireb2 ) ( c , d ), Fth1 ( e ), Ftl1 ( f ) and FERRITIN ( g ). Representative Western blot image of all the analyzed proteins ( h ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or by ACTIN protein levels and expressed as mean ± SEM; (* p < 0.05, ** p < 0.01 and *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. ISO; && p < 0.01 vs. DEF).

Techniques Used: Western Blot, Control

Isoproterenol modifies molecules involved in intracellular iron uptake in H9c2 cells. Analysis of mRNA ( a , c ) and protein ( b , d , e ) levels of TFRC ( a , b ) and DMT1 ( c , d ). Representative Western blot image of all the analyzed proteins ( e ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or ACTIN protein levels and expressed as mean ± SEM; (** p < 0.01 and *** p < 0.001 vs. control).

Figure Legend Snippet: Isoproterenol modifies molecules involved in intracellular iron uptake in H9c2 cells. Analysis of mRNA ( a , c ) and protein ( b , d , e ) levels of TFRC ( a , b ) and DMT1 ( c , d ). Representative Western blot image of all the analyzed proteins ( e ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or ACTIN protein levels and expressed as mean ± SEM; (** p < 0.01 and *** p < 0.001 vs. control).

Techniques Used: Western Blot, Control

Isoproterenol modifies molecules involved in intracellular iron release in H9c2 cells. Analysis of mRNA ( a , c ) and protein levels ( b , d , e ) of FPN ( a , b ) and HEPCIDIN ( c , d ). Representative Western blot image of all the analyzed proteins ( e ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or ACTIN protein levels and expressed as mean ± SEM; (* p < 0.05, ** p < 0.01 and *** p < 0.001 vs. control; ### p < 0.001 vs. ISO and &&& p < 0.001 vs. DEF).

Figure Legend Snippet: Isoproterenol modifies molecules involved in intracellular iron release in H9c2 cells. Analysis of mRNA ( a , c ) and protein levels ( b , d , e ) of FPN ( a , b ) and HEPCIDIN ( c , d ). Representative Western blot image of all the analyzed proteins ( e ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or ACTIN protein levels and expressed as mean ± SEM; (* p < 0.05, ** p < 0.01 and *** p < 0.001 vs. control; ### p < 0.001 vs. ISO and &&& p < 0.001 vs. DEF).

Techniques Used: Western Blot, Control

Isoproterenol modifies the expression of molecules involved in mitochondrial iron uptake and release in H9c2 cells. Analysis of Ftmt, Mfrn1, Mfrn2, Abcb7 and Abcb8 mRNA ( a , c , e , g , i ) and protein levels ( b , d , f , h , j ). Representative Western blot image of all the analyzed proteins ( k ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or by ACTIN protein levels and expressed as mean ± SEM; (* p < 0.05, ** p < 0.01 and *** p < 0.001 vs. control; # p < 0.05 and ## p < 0.01 vs. ISO).

Figure Legend Snippet: Isoproterenol modifies the expression of molecules involved in mitochondrial iron uptake and release in H9c2 cells. Analysis of Ftmt, Mfrn1, Mfrn2, Abcb7 and Abcb8 mRNA ( a , c , e , g , i ) and protein levels ( b , d , f , h , j ). Representative Western blot image of all the analyzed proteins ( k ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or by ACTIN protein levels and expressed as mean ± SEM; (* p < 0.05, ** p < 0.01 and *** p < 0.001 vs. control; # p < 0.05 and ## p < 0.01 vs. ISO).

Techniques Used: Expressing, Western Blot, Control

Isoproterenol downregulates mitofusin 1 and 2 expressions in H9c2 cells. Analysis of Mfn1 and Mfn2 mRNA ( a , c ) and protein levels ( b , d ). Representative Western blot image of all the analyzed proteins ( e ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or ACTIN protein levels and expressed as mean ± SEM; (* p < 0.05 and *** p < 0.001 vs. control; # p < 0.05 vs. ISO).

Figure Legend Snippet: Isoproterenol downregulates mitofusin 1 and 2 expressions in H9c2 cells. Analysis of Mfn1 and Mfn2 mRNA ( a , c ) and protein levels ( b , d ). Representative Western blot image of all the analyzed proteins ( e ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or ACTIN protein levels and expressed as mean ± SEM; (* p < 0.05 and *** p < 0.001 vs. control; # p < 0.05 vs. ISO).

Techniques Used: Western Blot, Control

Image Search Results

Journal: Biomolecules

Article Title: Iron Matters: Comparative Impact of Beta-Adrenergic Stimulation and Iron Chelation on Cardiac Iron Metabolism and Mitochondrial Function

doi: 10.3390/biom16040582

Figure Lengend Snippet: Isoproterenol depletes intracellular iron in H9c2 cells. Analysis of intracellular iron levels from the cells challenged with isoproterenol and/or deferoxamine. Data are expressed as mean ± SEM; (*** p < 0.001 vs. control).

Article Snippet: Rat embryonic heart-derived H9c2 cells (from ATCC-Massanas, VA, USA; CRL-1446) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; 4.5 g/L glucose), supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C in a humidified incubator with 95% air and 5% CO 2 [ ].

Techniques: Control

Journal: Biomolecules

Article Title: Iron Matters: Comparative Impact of Beta-Adrenergic Stimulation and Iron Chelation on Cardiac Iron Metabolism and Mitochondrial Function

doi: 10.3390/biom16040582

Figure Lengend Snippet: Isoproterenol modifies iron metabolism-related molecules in H9c2 cells. Analysis of mRNA ( a , c , e , f ) and protein levels ( b , d , g , h ) of IRP1 ( Aco1 ) ( a , b ), IRP2 ( Ireb2 ) ( c , d ), Fth1 ( e ), Ftl1 ( f ) and FERRITIN ( g ). Representative Western blot image of all the analyzed proteins ( h ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or by ACTIN protein levels and expressed as mean ± SEM; (* p < 0.05, ** p < 0.01 and *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. ISO; && p < 0.01 vs. DEF).

Techniques: Western Blot, Control

Journal: Biomolecules

Article Title: Iron Matters: Comparative Impact of Beta-Adrenergic Stimulation and Iron Chelation on Cardiac Iron Metabolism and Mitochondrial Function

doi: 10.3390/biom16040582

Figure Lengend Snippet: Isoproterenol modifies molecules involved in intracellular iron uptake in H9c2 cells. Analysis of mRNA ( a , c ) and protein ( b , d , e ) levels of TFRC ( a , b ) and DMT1 ( c , d ). Representative Western blot image of all the analyzed proteins ( e ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or ACTIN protein levels and expressed as mean ± SEM; (** p < 0.01 and *** p < 0.001 vs. control).

Techniques: Western Blot, Control

Journal: Biomolecules

Article Title: Iron Matters: Comparative Impact of Beta-Adrenergic Stimulation and Iron Chelation on Cardiac Iron Metabolism and Mitochondrial Function

doi: 10.3390/biom16040582

Figure Lengend Snippet: Isoproterenol modifies molecules involved in intracellular iron release in H9c2 cells. Analysis of mRNA ( a , c ) and protein levels ( b , d , e ) of FPN ( a , b ) and HEPCIDIN ( c , d ). Representative Western blot image of all the analyzed proteins ( e ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or ACTIN protein levels and expressed as mean ± SEM; (* p < 0.05, ** p < 0.01 and *** p < 0.001 vs. control; ### p < 0.001 vs. ISO and &&& p < 0.001 vs. DEF).

Techniques: Western Blot, Control

Journal: Biomolecules

Article Title: Iron Matters: Comparative Impact of Beta-Adrenergic Stimulation and Iron Chelation on Cardiac Iron Metabolism and Mitochondrial Function

doi: 10.3390/biom16040582

Figure Lengend Snippet: Isoproterenol modifies the expression of molecules involved in mitochondrial iron uptake and release in H9c2 cells. Analysis of Ftmt, Mfrn1, Mfrn2, Abcb7 and Abcb8 mRNA ( a , c , e , g , i ) and protein levels ( b , d , f , h , j ). Representative Western blot image of all the analyzed proteins ( k ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or by ACTIN protein levels and expressed as mean ± SEM; (* p < 0.05, ** p < 0.01 and *** p < 0.001 vs. control; # p < 0.05 and ## p < 0.01 vs. ISO).

Techniques: Expressing, Western Blot, Control

Journal: Biomolecules

Article Title: Iron Matters: Comparative Impact of Beta-Adrenergic Stimulation and Iron Chelation on Cardiac Iron Metabolism and Mitochondrial Function

doi: 10.3390/biom16040582

Figure Lengend Snippet: Isoproterenol downregulates mitofusin 1 and 2 expressions in H9c2 cells. Analysis of Mfn1 and Mfn2 mRNA ( a , c ) and protein levels ( b , d ). Representative Western blot image of all the analyzed proteins ( e ). Western blot original images can be found in . Data were normalized by the Gapdh mRNA or ACTIN protein levels and expressed as mean ± SEM; (* p < 0.05 and *** p < 0.001 vs. control; # p < 0.05 vs. ISO).

Techniques: Western Blot, Control

A-MG sustains viability and inhibits apoptosis in HG/F-induced H9C2 cells. (A) Results of CCK8 assays evaluating the viability of H9C2 cells exposed to high glucose and different concentrations of PA. (B) Results of CCK8 assays in HG/F-induced H9C2 cells treated with different concentrations of A-MG. (C) LDH release assay results. (D) Assessment of apoptotic damage in H9C2 cells by Hoechst 33258 staining. (E, F) Flow cytometry analysis of apoptosis in Annexin V/FITC-PI-labeled H9C2 cells. (G–K) Western blot analysis of BCL-2, BAX, cleaved caspase-3, cleaved caspase-9, and caspase-9 protein expressions in the NC, HG/F, and A-MG groups. The results represent at least three independent experiments; data are presented as the mean ± SD (n ≥ 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: α-Mangostin prevents diabetic cardiomyopathy by inhibiting oxidative damage and lipotoxicity through the AKT–FOXO1–CD36 pathway

doi: 10.3389/fphar.2025.1566311

Figure Lengend Snippet: A-MG sustains viability and inhibits apoptosis in HG/F-induced H9C2 cells. (A) Results of CCK8 assays evaluating the viability of H9C2 cells exposed to high glucose and different concentrations of PA. (B) Results of CCK8 assays in HG/F-induced H9C2 cells treated with different concentrations of A-MG. (C) LDH release assay results. (D) Assessment of apoptotic damage in H9C2 cells by Hoechst 33258 staining. (E, F) Flow cytometry analysis of apoptosis in Annexin V/FITC-PI-labeled H9C2 cells. (G–K) Western blot analysis of BCL-2, BAX, cleaved caspase-3, cleaved caspase-9, and caspase-9 protein expressions in the NC, HG/F, and A-MG groups. The results represent at least three independent experiments; data are presented as the mean ± SD (n ≥ 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Article Snippet: H9C2 embryonic rat heart-derived cell lines, obtained from Procell (CL-0089, Wuhan, China), were grown and maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, United States) containing 10% fetal bovine serum (FBS, C04001-500, VivaCell, China) at 37°C with 5% CO 2 .

Techniques: Lactate Dehydrogenase Assay, Staining, Flow Cytometry, Labeling, Western Blot

A-MG alleviates oxidative stress in HG/F-induced H9C2 cells. (A) Flow cytometry analysis of intracellular ROS generation in DCFH-DA-loaded H9C2 cells. (B) Quantification of MDA levels; data were normalized to the control group. (C, D) Representative images of JC-1-stained H9C2 cells (C) and corresponding quantitative analysis (D) . (E) SOD activity measurements in H9C2 cells. (F) RT-qPCR detection of Nrf2, HO-1, and SOD2 mRNA levels. (G–J) Western blot images and corresponding analysis of Nrf2, HO-1, and SOD2 expression in H9C2 cells. The results represent at least three independent experiments; data are presented as the mean ± SD (n ≥ 3). * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Frontiers in Pharmacology

Article Title: α-Mangostin prevents diabetic cardiomyopathy by inhibiting oxidative damage and lipotoxicity through the AKT–FOXO1–CD36 pathway

doi: 10.3389/fphar.2025.1566311

Figure Lengend Snippet: A-MG alleviates oxidative stress in HG/F-induced H9C2 cells. (A) Flow cytometry analysis of intracellular ROS generation in DCFH-DA-loaded H9C2 cells. (B) Quantification of MDA levels; data were normalized to the control group. (C, D) Representative images of JC-1-stained H9C2 cells (C) and corresponding quantitative analysis (D) . (E) SOD activity measurements in H9C2 cells. (F) RT-qPCR detection of Nrf2, HO-1, and SOD2 mRNA levels. (G–J) Western blot images and corresponding analysis of Nrf2, HO-1, and SOD2 expression in H9C2 cells. The results represent at least three independent experiments; data are presented as the mean ± SD (n ≥ 3). * p < 0.05, ** p < 0.01, and *** p < 0.001.

Techniques: Flow Cytometry, Control, Staining, Activity Assay, Quantitative RT-PCR, Western Blot, Expressing

A-MG reduces lipid accumulation in HG/F-induced H9C2 cells. (A) Heatmap depicting A-MG-induced changes in gene expression influencing fat digestion and absorption. (B) GSEA gene enrichment map. (C) RT-qPCR analysis of CD36, PPARα, and CPT1β mRNA levels in H9C2 cells. (D) Representative images of intracellular lipid droplets in H9C2 cells stained with BODIPY 493/503. (E) Quantification of the mean fluorescence intensity of BODIPY 493/503; IntDen/Area (A.U.), integrated density/area (arbitrary unit). (F–H) Western blot analysis of p-FOXO1, FOXO1, and CD36 protein expressions in H9C2 cells. (I) Representative images of immunofluorescent staining of FOXO1 in H9C2 cells. (J) Quantification of the FOXO1 immunofluorescence signal in H9C2 cells. (K–M) Analysis of docking between A-MG and CD36 proteins. (K) 3D structure of CD36 bound with A-MG; the structure of CD36 is shown in cyan and A-MG is rendered in green. (L) Depiction of the electrostatic surface of the A-MG binding site on CD36. (M) Detail of the binding mode of A-MG on CD36. The results represent at least three independent experiments; data are expressed as the mean ± SD (n ≥ 3). * p < 0.05, ** p < 0.01, and *** p < 0.001.

Journal: Frontiers in Pharmacology

Article Title: α-Mangostin prevents diabetic cardiomyopathy by inhibiting oxidative damage and lipotoxicity through the AKT–FOXO1–CD36 pathway

doi: 10.3389/fphar.2025.1566311

Figure Lengend Snippet: A-MG reduces lipid accumulation in HG/F-induced H9C2 cells. (A) Heatmap depicting A-MG-induced changes in gene expression influencing fat digestion and absorption. (B) GSEA gene enrichment map. (C) RT-qPCR analysis of CD36, PPARα, and CPT1β mRNA levels in H9C2 cells. (D) Representative images of intracellular lipid droplets in H9C2 cells stained with BODIPY 493/503. (E) Quantification of the mean fluorescence intensity of BODIPY 493/503; IntDen/Area (A.U.), integrated density/area (arbitrary unit). (F–H) Western blot analysis of p-FOXO1, FOXO1, and CD36 protein expressions in H9C2 cells. (I) Representative images of immunofluorescent staining of FOXO1 in H9C2 cells. (J) Quantification of the FOXO1 immunofluorescence signal in H9C2 cells. (K–M) Analysis of docking between A-MG and CD36 proteins. (K) 3D structure of CD36 bound with A-MG; the structure of CD36 is shown in cyan and A-MG is rendered in green. (L) Depiction of the electrostatic surface of the A-MG binding site on CD36. (M) Detail of the binding mode of A-MG on CD36. The results represent at least three independent experiments; data are expressed as the mean ± SD (n ≥ 3). * p < 0.05, ** p < 0.01, and *** p < 0.001.

Techniques: Gene Expression, Quantitative RT-PCR, Staining, Fluorescence, Western Blot, Immunofluorescence, Binding Assay

A-MG decreases fatty acid excessive β-oxidation in HG/F-induced H9C2 cells. (A–D) Western blot analysis of PPARα, CPT1β, and ACADM protein levels in H9C2 cells. (E–G) After lentiviral transduction, analysis of FOXO1 mRNA (E) and protein (F) expression in H9C2 cells; quantitative data are shown in (G) . (H) GFP fluorescence measurements in H9C2 cells transduced with FOXO-OE vectors. The data are presented as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. (I–L) Western blot analysis of the effect of FOXO1 overexpression on PPARα, CPT1β, and ACADM expression in the five H9C2 cell groups. The results represent at least three independent experiments; data are expressed as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01.

Journal: Frontiers in Pharmacology

Article Title: α-Mangostin prevents diabetic cardiomyopathy by inhibiting oxidative damage and lipotoxicity through the AKT–FOXO1–CD36 pathway

doi: 10.3389/fphar.2025.1566311

Figure Lengend Snippet: A-MG decreases fatty acid excessive β-oxidation in HG/F-induced H9C2 cells. (A–D) Western blot analysis of PPARα, CPT1β, and ACADM protein levels in H9C2 cells. (E–G) After lentiviral transduction, analysis of FOXO1 mRNA (E) and protein (F) expression in H9C2 cells; quantitative data are shown in (G) . (H) GFP fluorescence measurements in H9C2 cells transduced with FOXO-OE vectors. The data are presented as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. (I–L) Western blot analysis of the effect of FOXO1 overexpression on PPARα, CPT1β, and ACADM expression in the five H9C2 cell groups. The results represent at least three independent experiments; data are expressed as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01.

Techniques: Western Blot, Transduction, Expressing, Fluorescence, Over Expression

A-MG activates the AKT pathway. (A–E) Western blot analysis examining the effect of LY294002 on AKT, p-AKT, Nrf2, SOD2, and HO-1 protein expression in HG/F-induced H9C2 cells. (F–I) Western blot analysis examining the effect of LY294002 on FOXO1, p-FOXO1, CD36, and CPT1β expression in HG/F-induced H9C2 cells. The results represent at least three independent experiments; data are expressed as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: α-Mangostin prevents diabetic cardiomyopathy by inhibiting oxidative damage and lipotoxicity through the AKT–FOXO1–CD36 pathway

doi: 10.3389/fphar.2025.1566311

Figure Lengend Snippet: A-MG activates the AKT pathway. (A–E) Western blot analysis examining the effect of LY294002 on AKT, p-AKT, Nrf2, SOD2, and HO-1 protein expression in HG/F-induced H9C2 cells. (F–I) Western blot analysis examining the effect of LY294002 on FOXO1, p-FOXO1, CD36, and CPT1β expression in HG/F-induced H9C2 cells. The results represent at least three independent experiments; data are expressed as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Techniques: Western Blot, Expressing

(A) Concentration of 18 F-FDG taken up by the hearts of patients treated with chemotherapy and without chemotherapy. (B) 18 F-FDG PET/CT image acquisition and cardiac 18 F-FDG uptake. (C) Heat map of Dox-treated mice. (D) MDA content in H9c2 cells. (E) HE staining showing that Dox reduced the cardiomyocyte size. Control vs. Dox: *** p < 0.0005, ** p < 0.005, * p < 0.05, mean ± SD. n = 6–9.

Journal: Frontiers in Pharmacology

Article Title: Breviscapine remodels myocardial glucose and lipid metabolism by regulating serotonin to alleviate doxorubicin-induced cardiotoxicity

doi: 10.3389/fphar.2022.930835

Figure Lengend Snippet: (A) Concentration of 18 F-FDG taken up by the hearts of patients treated with chemotherapy and without chemotherapy. (B) 18 F-FDG PET/CT image acquisition and cardiac 18 F-FDG uptake. (C) Heat map of Dox-treated mice. (D) MDA content in H9c2 cells. (E) HE staining showing that Dox reduced the cardiomyocyte size. Control vs. Dox: *** p < 0.0005, ** p < 0.005, * p < 0.05, mean ± SD. n = 6–9.

Article Snippet: H9c2 embryonic rat heart-derived cardiomyoblast cells (ATCC, CRL-1446) were cultured in DMEM containing 10% fetal bovine serum, 100 units/ml penicillin G sodium, and 100 μg/ml streptomycin sulfate (37°C, 5% CO 2 ).

Techniques: Concentration Assay, Positron Emission Tomography-Computed Tomography, Staining, Control

(A) Intensity of ROS staining in H9c2 cells. (B) Cardiac oxygen consumption in H9c2 cells. (C) The mitochondrial membrane potential. (D) JC-1 staining and bar graph. (E) ATP content in H9c2 cells. (F) The ATP production/oxygen consumption ratio in H9c2 cells. (G) The expression levels of AMPK and β-tubulin were measured in vitro by WB. Control vs. Dox: *** p < 0.0005, ** p < 0.005, * p < 0.05, mean ± SD. n = 6–9.

Journal: Frontiers in Pharmacology

Article Title: Breviscapine remodels myocardial glucose and lipid metabolism by regulating serotonin to alleviate doxorubicin-induced cardiotoxicity

doi: 10.3389/fphar.2022.930835

Figure Lengend Snippet: (A) Intensity of ROS staining in H9c2 cells. (B) Cardiac oxygen consumption in H9c2 cells. (C) The mitochondrial membrane potential. (D) JC-1 staining and bar graph. (E) ATP content in H9c2 cells. (F) The ATP production/oxygen consumption ratio in H9c2 cells. (G) The expression levels of AMPK and β-tubulin were measured in vitro by WB. Control vs. Dox: *** p < 0.0005, ** p < 0.005, * p < 0.05, mean ± SD. n = 6–9.

Techniques: Staining, Membrane, Expressing, In Vitro, Control

(A) ATP content in H9c2 cells. (B) MDA content in H9c2 cells. (C) Cardiac oxygen consumption in H9c2 cells. (D) The ATP production/oxygen consumption ratio in H9c2 cells. (E) The intensity of ROS staining in H9c2 cells. (F) Schematic diagram. Control vs. Dox, Brev vs. Dox, and Dexra vs. Dox: *** p < 0.0005, ** p < 0.005, * p < 0.05, mean ± SD. n = 6–9.

Journal: Frontiers in Pharmacology

Article Title: Breviscapine remodels myocardial glucose and lipid metabolism by regulating serotonin to alleviate doxorubicin-induced cardiotoxicity

doi: 10.3389/fphar.2022.930835

Figure Lengend Snippet: (A) ATP content in H9c2 cells. (B) MDA content in H9c2 cells. (C) Cardiac oxygen consumption in H9c2 cells. (D) The ATP production/oxygen consumption ratio in H9c2 cells. (E) The intensity of ROS staining in H9c2 cells. (F) Schematic diagram. Control vs. Dox, Brev vs. Dox, and Dexra vs. Dox: *** p < 0.0005, ** p < 0.005, * p < 0.05, mean ± SD. n = 6–9.

Techniques: Staining, Control

(A) Expression levels of PINK1, Parkin, Tom20, and GAPDH were measured in vitro by WB. (B) The expression levels of p-PINK1, p-Parkin, and Tom20 were assessed. (C) Mitotracer staining. (D) Electron microscopy. (E) The expression levels of p-AMPK, p-mTOR, p-Akt, and p-PI3K were evaluated. (F) The intensity of ROS staining in H9c2 cells after supplementation with Mdivi-1. (G) ATP content in H9c2 cells after supplementation with Mdivi-1. (H) Bar graph of p-AMPK/t-AMPK, p-mTOR/t-mTOR, p-Akt/t-Akt, and p-PI3K/t-PI3K. Control vs. Dox, Dox + Mdivi-1 vs. Dox, and Dox + Brev + Mdivi-1 vs. Dox + Brev: *** p < 0.0005, ** p < 0.005, * p < 0.05, mean ± SD. n = 6–9.

Journal: Frontiers in Pharmacology

Article Title: Breviscapine remodels myocardial glucose and lipid metabolism by regulating serotonin to alleviate doxorubicin-induced cardiotoxicity

doi: 10.3389/fphar.2022.930835

Figure Lengend Snippet: (A) Expression levels of PINK1, Parkin, Tom20, and GAPDH were measured in vitro by WB. (B) The expression levels of p-PINK1, p-Parkin, and Tom20 were assessed. (C) Mitotracer staining. (D) Electron microscopy. (E) The expression levels of p-AMPK, p-mTOR, p-Akt, and p-PI3K were evaluated. (F) The intensity of ROS staining in H9c2 cells after supplementation with Mdivi-1. (G) ATP content in H9c2 cells after supplementation with Mdivi-1. (H) Bar graph of p-AMPK/t-AMPK, p-mTOR/t-mTOR, p-Akt/t-Akt, and p-PI3K/t-PI3K. Control vs. Dox, Dox + Mdivi-1 vs. Dox, and Dox + Brev + Mdivi-1 vs. Dox + Brev: *** p < 0.0005, ** p < 0.005, * p < 0.05, mean ± SD. n = 6–9.

Techniques: Expressing, In Vitro, Staining, Electron Microscopy, Control

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